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KMID : 0359319930330020285
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Korean Journal of Veterinary Research
1993 Volume.33 No. 2 p.285 ~ p.287
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Preservation of Toxoplasma gondii ( RH strain ) in liquid nitrogen for 5 years
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Abstract
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Toxoplasma gondii frozen by the method of very slow cooling (-30℃/3hrs.→-60℃ /lhr.→-196℃ of liquid nitrogen)were inoculated mice after storage in the liquid nitrogen for 5 years.
The results obtained were as follows ;
1. Toxoplasma gondii was detected in mice 4∼5th day after intraperitoneal inoculation of organisms stored in the liquid nitrogen once a year for 5 years.
2. Toxoplasma gondii could be preserved longer than 5 years in liquid nitorgen being evidenced by the in vivo test.
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KEYWORD
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